| Primary Identifier | MGI:2154542 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Hmx2 |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Insertion of an IRES-lacZ-neomycin resistance cassette into exon 2, which encodes the third helix of the homeodomain, introduced premature translation stop codons in all three open reading frames. This allele produces beta-galactosidase and neomycin phosphotransferase under the control of the endogenous promoter. The authors predict that this allele would also produce a truncated protein that lacks the carboxy terminus. RNase protection analysis did not detect wild-type homeobox in homozygous mutant embryos. |
