Primary Identifier | MGI:2154694 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Sgcd |
Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A neomycin resistance cassette replaced exon 2, which encodes 63 amino acids of the intracellular domain and the entire transmembrane domain. Northern blot analysis revealed that a transcript lacking exon 2 sequences is produced from this allele, but no protein was detected in skeletal muscle membrane enriched preparations of homozygous mice by western blot analysis. |