| First Author | Bruurs LJM | Year | 2018 |
| Journal | Mol Cell Biol | PubMed ID | 29581186 |
| Mgi Jnum | J:265267 | Mgi Id | MGI:6199224 |
| Doi | 10.1128/MCB.00102-18 | Citation | Bruurs LJM, et al. (2018) The phosphatase PTPL1 is required for PTEN-mediated regulation of apical membrane size. Mol Cell Biol |
| abstractText | PTEN is a tumor suppressor frequently lost in epithelial malignancies. Part of the tumor suppressive properties of PTEN is attributed to its function in cell polarization and consequently its role in maintaining epithelial tissue integrity. However, surprisingly little is known about the function and regulation of PTEN during epithelial cell polarization.We used CRISPR/Cas9-mediated gene disruption to delete PTEN in intestinal epithelial Ls174T:W4 cells, which upon differentiation form a microvilli-covered apical membrane (brush border) on a part of the cell cortex, independent of cell-cell junctions. We show that loss of PTEN results in the formation of a larger brush border that, in a fraction of the cells, even spans the entire plasma membrane, revealing that PTEN functions in the regulation of apical membrane size. Depletion of the phosphatase PTPL1 resulted in a similar defect. PTPL1 interacts with PTEN, and this interaction is necessary for apical membrane enrichment of PTEN. Importantly, phosphatase activity of PTPL1 is not required, indicating that PTPL1 functions as an anchor protein in this process.Our work thus demonstrates a novel function for PTEN during cell polarization in controlling apical membrane size and identifies PTPL1 as a critical apical membrane anchor for PTEN in this process. |
