| First Author | Takayama S | Year | 2023 |
| Journal | Sci Rep | Volume | 13 |
| Issue | 1 | Pages | 519 |
| PubMed ID | 36627369 | Mgi Jnum | J:334793 |
| Mgi Id | MGI:7427041 | Doi | 10.1038/s41598-023-27737-7 |
| Citation | Takayama S, et al. (2023) Angiotensin II type 1a receptor deficiency alleviates muscle atrophy after denervation. Sci Rep 13(1):519 |
| abstractText | The study aim was to determine if suppressed activation of angiotensin II type 1 receptor (AT1) prevents severe muscle atrophy after denervation. The sciatic nerves in right and left inferior limbs were cut in AT1a knockout homo (AT1a(-/-)) male mice and wild-type (AT1a(+/+)) male mice. Muscle weight and cross-sectional areas of type IIb muscle fibers in gastrocnemius muscle decreased at 7 and 21 days postdenervation in both AT1a(-/-) mice and AT1a(+/+) mice, and the reduction was significantly attenuated in the denervated muscles of AT1a(-/-) mice compared to the AT1a(+/+) mice. Gene expressions in the protein degradation system [two E3 ubiquitin ligases (muscle RING-finger protein-1 and Atrogin-1)] upregulated at 7 days postdenervation in all denervated mice were significantly lower in AT1a(-/-) mice than in AT1a(+/+) mice. Activations of nuclear factor kappaB and Forkhead box subgroup O1, and protein expression of monocyte chemoattractant protein-1 were significantly suppressed in the AT1a(-/-) mice compared with those in the AT1a(+/+) mice. In addition, suppressed apoptosis, lower infiltration of M1 macrophages, and higher infiltration of M2 macrophages were significantly observed at 21 days postdenervation in the AT1a(-/-) mice compared with those in the AT1a(+/+) mice. In conclusion, the AT1 receptor deficiency retarded muscle atrophy after denervation. |
