| First Author | Lee PP | Year | 2001 |
| Journal | Immunity | Volume | 15 |
| Issue | 5 | Pages | 763-74 |
| PubMed ID | 11728338 | Mgi Jnum | J:73127 |
| Mgi Id | MGI:2154601 | Doi | 10.1016/s1074-7613(01)00227-8 |
| Citation | Lee PP, et al. (2001) A critical role for dnmt1 and DNA methylation in T cell development, function, and survival. Immunity 15(5):763-74 |
| abstractText | The role of DNA methylation and of the maintenance DNA methyltransferase Dnmt1 in the epigenetic regulation of developmental stage- and cell lineage-specific gene expression in vivo is uncertain. This is addressed here through the generation of mice in which Dnmt1 was inactivated by Cre/loxP-mediated deletion at sequential stages of T cell development. Deletion of Dnmt1 in early double-negative thymocytes led to impaired survival of TCRalphabeta(+) cells and the generation of atypical CD8(+)TCRgammadelta(+) cells. Deletion of Dnmt1 in double-positive thymocytes impaired activation-induced proliferation but differentially enhanced cytokine mRNA expression by naive peripheral T cells. We conclude that Dnmt1 and DNA methylation are required for the proper expression of certain genes that define fate and determine function in T cells. |
