| First Author | Zimmerman KA | Year | 2018 |
| Journal | Am J Physiol Gastrointest Liver Physiol | Volume | 314 |
| Issue | 6 | Pages | G677-G689 |
| PubMed ID | 29543508 | Mgi Jnum | J:273395 |
| Mgi Id | MGI:6281496 | Doi | 10.1152/ajpgi.00381.2017 |
| Citation | Zimmerman KA, et al. (2018) Primary cilia disruption differentially affects the infiltrating and resident macrophage compartment in the liver. Am J Physiol Gastrointest Liver Physiol 314(6):G677-G689 |
| abstractText | Hepatorenal fibrocystic disease (HRFCD) is characterized by cysts in the kidney and liver with associated fibrosis and is the result of defects in proteins required for cilia function or assembly. Previous reports indicate that macrophages, mainly M2-like macrophages, contribute to HRFCD, although the origin of these cells (yolk sac-derived resident macrophages vs. bone marrow-derived infiltrating macrophages) and their contribution to the observed phenotypes are unknown. We utilize a congenital model of cilia dysfunction (IFT88(Orpk)) to study the importance of macrophages in HRFCD. Our data show a rapid expansion of the bile duct region and development of fibrosis between 2 and 4 wk of age. Immunofluorescence microscopy analysis reveals an accumulation of F4/80(+) macrophages in regions exhibiting biliary hyperplasia in IFT88(Orpk) mice. Flow cytometry data show that cilia dysfunction leads to an accumulation of infiltrating macrophages (CD11b(hi), F4/80(lo)) and a reduction of resident macrophage (CD11b(lo), F4/80(hi)) number. A majority of the infiltrating macrophages are Ly6c(hi) profibrogenic macrophages. Along with the accumulation of immune cells, expression of proinflammatory and profibrotic transcripts, including TGF-beta, TNF-alpha, IL-1beta, and chemokine (C-C) motif ligand 2, is increased. Quantitative RT-PCR analysis of flow-sorted cells shows enhanced expression of CCL2 in cholangiocytes and enhanced expression of VEGF-A and IL-6 in Ly6c(hi) macrophages. Genetic inhibition of Ly6c(hi) macrophage accumulation in IFT88(Orpk) FVB CCR2(-/-) mice reduced biliary fibrosis but did not affect epithelial expansion. Collectively, these studies suggest that biliary epithelium with defects in primary cilia preferentially recruits Ly6c(hi) infiltrating macrophages, which promote fibrotic progression in HRFCD pathogenesis. NEW & NOTEWORTHY These studies are the first to address the contribution of the infiltrating and resident macrophage niche during progression of hepatorenal fibrocystic disease (HRFCD). We show that the number of infiltrating macrophages is significantly upregulated in HRFCD mouse models. Finally, we show that prevention of Ly6c(hi) infiltrating macrophage accumulation significantly reduces biliary fibrosis, but not biliary hyperplasia, suggesting that this population may be responsible for the fibrotic progression of the disease in HRFCD patients. |
