| First Author | Lohmann F | Year | 2015 |
| Journal | Mol Cell Biol | Volume | 35 |
| Issue | 21 | Pages | 3726-38 |
| PubMed ID | 26303528 | Mgi Jnum | J:228214 |
| Mgi Id | MGI:5705676 | Doi | 10.1128/MCB.00382-15 |
| Citation | Lohmann F, et al. (2015) The DEK Oncoprotein Is a Critical Component of the EKLF/KLF1 Enhancer in Erythroid Cells. Mol Cell Biol 35(21):3726-38 |
| abstractText | Understanding how transcriptional regulators are themselves controlled is important in attaining a complete picture of the intracellular effects that follow signaling cascades during early development and cell-restricted differentiation. We have addressed this issue by focusing on the regulation of EKLF/KLF1, a zinc finger transcription factor that plays a necessary role in the global regulation of erythroid gene expression. Using biochemical affinity purification, we have identified the DEK oncoprotein as a critical factor that interacts with an essential upstream enhancer element of the EKLF promoter and exerts a positive effect on EKLF levels. This element also binds a core set of erythroid transcription factors, suggesting that DEK is part of a tissue-restricted enhanceosome that contains BMP4-dependent and -independent components. Together with local enrichment of properly coded histones and an open chromatin domain, optimal transcriptional activation of the EKLF locus can be established. |
