| First Author | Sasada T | Year | 2001 |
| Journal | J Immunol | Volume | 166 |
| Issue | 4 | Pages | 2394-403 |
| PubMed ID | 11160298 | Mgi Jnum | J:126138 |
| Mgi Id | MGI:3760588 | Doi | 10.4049/jimmunol.166.4.2394 |
| Citation | Sasada T, et al. (2001) A critical role for CD2 in both thymic selection events and mature T cell function. J Immunol 166(4):2394-403 |
| abstractText | To examine the function of CD2 in vivo, N15 TCR transgenic (tg) RAG-2(-/-) H-2(b) mice bearing a single TCR specific for the vesicular stomatitis virus octapeptide bound to the H-2K(b) molecule were compared on a wild-type or CD2(-/-) background. In N15tg RAG-2(-/-) CD2(-/-) mice, thymic dysfunction is evident by 6 wk with a pre-TCR block in the CD4(-)CD8(-) double-negative thymocytes at the CD25(+)CD44(-) stage. Moreover, mature N15tg RAG-2(-/-) CD2(-/-) T cells are approximately 100-fold less responsive to vesicular stomatitis virus octapeptide and unresponsive to weak peptide agonists, as judged by IFN-gamma production. Repertoire analysis shows substantial differences in Valpha usage between non-tg C57BL/6 (B6) and B6 CD2(-/-) mice. Collectively, these findings show that CD2 plays a role in pre-TCR function in double-negative thymocytes, TCR selection events during thymocyte development, and TCR-stimulated cytokine production in mature T cells. |
