Primary Identifier | MGI:2158353 | Allele Type | Targeted |
Attribute String | Conditional ready, No functional change | Gene | Mecp2 |
Transmission | Germline | Strain of Origin | (C57BL/6 x 129S4/SvJae)F1 |
Is Recombinase | false | Is Wild Type | false |
molecularNote | LoxP sites were inserted flanking exon 3 as follows: A single loxP site was inserted 5' to exon 3 and a loxP-flanked hygromycin and thymidine kinase selection cassette was inserted 3' to exon 3. The selection cassette was removed in ES cells by Cre-mediated recombination prior to the production of chimeric mice, leaving a single loxP site in place of the selection cassette. |