| First Author | Shishikura M | Year | 2016 |
| Journal | Brain Res | Volume | 1642 |
| Pages | 244-254 | PubMed ID | 27026654 |
| Mgi Jnum | J:235871 | Mgi Id | MGI:5803896 |
| Doi | 10.1016/j.brainres.2016.03.030 | Citation | Shishikura M, et al. (2016) Expression of receptor protein tyrosine phosphatase delta, PTPdelta, in mouse central nervous system. Brain Res 1642:244-54 |
| abstractText | Protein tyrosine phosphate delta (PTPdelta), one of the receptor type IIa protein tyrosine phosphates, is known for its roles in axon guidance, synapse formation, cell adhesion, and tumor suppression. Alternative splicing of this gene generates at least four (A-D) isoforms; however, the major isoform in vivo is yet to be determined. The protein localization has neither been revealed. We have generated anti-mouse PTPdelta-specific monoclonal antibody and analyzed the protein expression in wild-type and Ptpdelta knockout mice. Immunoblot analysis of various organs revealed that neuronal tissues express both C-and D-isoforms of PTPdelta, whereas non-neuronal tissues express only C-isoform. Immunohistochemistry of wild-type or Ptpdelta heterozygous sections showed that olfactory bulb, cerebral cortex, hippocampus, cerebellum, and several nuclei in brain stem exhibit moderate to strong positive signals. These signals were absent in Ptpdelta knockout specimens. Higher magnification revealed differences between expression patterns of PTPdelta mRNA and its protein product. In hippocampus, weak mRNA expression in CA1 stratum pyramidale but strong immunostaining in the stratum lacunosum moleculare was observed, suggesting the axonal expression of PTPdelta in the entorhinal cortical afferents. Olfactory mitral cells exhibited mRNA expression in cell bodies and protein localization in their dendritic fields, glomerular and external plexiform layers. Nissl staining showed that the external plexiform layer was reduced in Ptpdelta knockout mice. Golgi-impregnation confirmed the poor dendritic growth of homozygous mitral cells. These results suggest that PTPdelta may localize in axons as well as in dendrites to regulate their elaboration in the central nervous system. |
