| First Author | Ye M | Year | 2013 |
| Journal | Nat Cell Biol | Volume | 15 |
| Issue | 4 | Pages | 385-94 |
| PubMed ID | 23502316 | Mgi Jnum | J:197635 |
| Mgi Id | MGI:5494206 | Doi | 10.1038/ncb2698 |
| Citation | Ye M, et al. (2013) C/EBPa controls acquisition and maintenance of adult haematopoietic stem cell quiescence. Nat Cell Biol 15(4):385-94 |
| abstractText | In blood, the transcription factor C/EBPa is essential for myeloid differentiation and has been implicated in regulating self-renewal of fetal liver haematopoietic stem cells (HSCs). However, its function in adult HSCs has remained unknown. Here, using an inducible knockout model we found that C/EBPa-deficient adult HSCs underwent a pronounced increase in number with enhanced proliferation, characteristics resembling fetal liver HSCs. Consistently, transcription profiling of C/EBPa-deficient HSCs revealed a gene expression program similar to fetal liver HSCs. Moreover, we observed that age-specific Cebpa expression correlated with its inhibitory effect on the HSC cell cycle. Mechanistically we identified N-Myc as a downstream target of C/EBPa, and loss of C/EBPa resulted in de-repression of N-Myc. Our data establish C/EBPa as a central determinant in the switch from fetal to adult HSCs. |
