| First Author | Madan V | Year | 2023 |
| Journal | Blood Adv | PubMed ID | 37611161 |
| Mgi Jnum | J:339863 | Mgi Id | MGI:7524654 |
| Doi | 10.1182/bloodadvances.2023009946 | Citation | Madan V, et al. (2023) Dissecting the role of SWI/SNF component ARID1B in steady state hematopoiesis. Blood Adv |
| abstractText | The ATP-dependent chromatin remodeling complex, SWI/SNF, has been implicated in normal hematopoiesis. The AT-Rich Interaction Domain 1B (ARID1B) and its paralog, ARID1A, are mutually exclusive, the DNA-interacting subunits of the BAF subclass of SWI/SNF complex. While the role of several SWI/SNF components in hematopoietic differentiation and stem cell maintenance has been reported, function of ARID1B in hematopoietic development has not been defined. To this end, we generated a mouse model of Arid1b-deficiency specifically in the hematopoietic compartment. Unlike the extensive phenotype observed in mice deficient in its paralog, ARID1A, Arid1b knockout mice exhibited a modest effect on steady-state hematopoiesis. Nonetheless, transplantation experiments showed that the reconstitution of myeloid cells in irradiated recipient mice was dependent on ARID1B. Further, to assess the effect of the complete loss of ARID1 proteins in the BAF complex, we generated mice lacking both ARID1A and ARID1B in the hematopoietic compartment. The double KO mice succumbed to acute bone marrow failure resulting from complete loss of BAF-mediated chromatin remodeling activity. Our ATAC-seq analyses revealed that >80% of loci regulated by ARID1B were distinct from those regulated by ARID1A; and ARID1B controlled expression of genes crucial in myelopoiesis. Overall, loss of ARID1B impacted chromatin dynamics in murine hematopoietic stem/progenitor cells, albeit to a lesser extent than cells lacking ARID1A. |
