| First Author | Zhang J | Year | 2013 |
| Journal | PLoS One | Volume | 8 |
| Issue | 12 | Pages | e82818 |
| PubMed ID | 24349371 | Mgi Jnum | J:211152 |
| Mgi Id | MGI:5574160 | Doi | 10.1371/journal.pone.0082818 |
| Citation | Zhang J, et al. (2013) Germ-line recombination activity of the widely used hGFAP-Cre and nestin-Cre transgenes. PLoS One 8(12):e82818 |
| abstractText | Herein we demonstrate with PCR, immunodetection and reporter gene approaches that the widely used human Glial Fibrillary Acidic Protein (hGFAP)-Cre transgene exhibits spontaneous germ-line recombination activity in leading to deletion in brain, heart and tail tissue with high frequency. The ectopic activity of hGFAP-Cre requires a rigorous control. We likewise observed that a second widely used nestin-Cre transgene shows germ-line deletion. Here we describe procedures to identify mice with germ-line recombination mediated by the hGFAP-Cre and nestin-Cre transgenes. Such control is essential to avoid pleiotropic effects due to germ-line deletion of loxP-flanked target genes and to maintain the CNS-restricted deletion status in transgenic mouse colonies. |
