| First Author | Loonen LM | Year | 2014 |
| Journal | Mucosal Immunol | Volume | 7 |
| Issue | 4 | Pages | 939-47 |
| PubMed ID | 24345802 | Mgi Jnum | J:331545 |
| Mgi Id | MGI:6835029 | Doi | 10.1038/mi.2013.109 |
| Citation | Loonen LM, et al. (2014) REG3gamma-deficient mice have altered mucus distribution and increased mucosal inflammatory responses to the microbiota and enteric pathogens in the ileum. Mucosal Immunol 7(4):939-47 |
| abstractText | REG3gamma is considered to have a protective role against infection with Gram-positive bacteria due to its bactericidal activity, but evidence from in vivo studies is lacking. We generated a REG3gamma(-/-) mouse, and investigated the effect of lack of REG3gamma on intestinal mucus distribution, spatial compartmentalization of bacteria, and expression of innate immunity genes. Infection studies were also performed with Gram-positive and Gram-negative pathogens to investigate the antimicrobial role of REG3gamma. REG3gamma(-/-) mice display altered mucus distribution, increased bacterial contact with the epithelium, and elevated inflammatory markers in the ileum without histological evidence of pathology. Infection response pathway genes were differentially expressed in both Listeria monocytogenes and Salmonella enteritidis infected REG3gamma(-/-) and wild-type (wt) mice. Higher amounts of myeloperoxidase and interleukin-22 transcripts were present in the ileal mucosa of REG3gamma(-/-) than wt mice, but translocation to the organs was unaffected. We concluded that REG3gamma has a protective role against mucosal infection with pathogenic Listeria and Salmonella in vivo. REG3gamma is equally distributed throughout the mucus and its absence results in increased epithelial contact with the microbiota resulting in low-grade inflammation. REG3gamma can bind to Gram-negative and Gram-positive bacteria and influence mucus distribution in the ileum, properties which may contribute to mucosal protection. |
