| First Author | Weissgerber P | Year | 2011 |
| Journal | Sci Signal | Volume | 4 |
| Issue | 171 | Pages | ra27 |
| PubMed ID | 21540454 | Mgi Jnum | J:185996 |
| Mgi Id | MGI:5430713 | Doi | 10.1126/scisignal.2001791 |
| Citation | Weissgerber P, et al. (2011) Male fertility depends on Ca(2)+ absorption by TRPV6 in epididymal epithelia. Sci Signal 4(171):ra27 |
| abstractText | TRPV6 [transient receptor potential vanilloid 6] is a calcium ion (Ca(2)+)-selective channel originally identified in the duodenal epithelium and in placenta; replacement of a negatively charged aspartate in the pore-forming region with an uncharged alanine (D541A) renders heterologously expressed TRPV6 channels nonfunctional. We found that male, but not female, mice homozygous for this mutation (Trpv6(D541A/D541A)) showed severely impaired fertility. The motility and fertilization capacity of sperm were markedly reduced, despite intact spermatogenesis. Trpv6 was expressed in epididymal epithelium where the protein was detected in the apical membrane, whereas it was not expressed in spermatozoa or the germinal epithelium. The Ca(2)+ concentration of the fluid in the cauda epididymis of Trpv6(D541A/D541A) males was 10 times higher than that of wild-type mice, which was accompanied by a seven- to eightfold decrease in Ca(2)+ absorption through the epididymal epithelium and was associated with reduced sperm viability. Thus, appropriate Ca(2)+ absorption and a consequent TRPV6-mediated decrease in the extracellular Ca(2)+ concentration toward the distal segments of the epididymal duct are essential for the acquisition of basic functions and the survival of spermatozoa. |
