| First Author | Kretzer NM | Year | 2016 |
| Journal | J Exp Med | Volume | 213 |
| Issue | 13 | Pages | 2871-2883 |
| PubMed ID | 27899443 | Mgi Jnum | J:237493 |
| Mgi Id | MGI:5812820 | Doi | 10.1084/jem.20160597 |
| Citation | Kretzer NM, et al. (2016) RAB43 facilitates cross-presentation of cell-associated antigens by CD8alpha+ dendritic cells. J Exp Med 213(13):2871-2883 |
| abstractText | In this study, to examine cross-presentation by classical dendritic cells (DCs; cDCs), we evaluated the role of RAB43, a protein found to be selectively expressed by Batf3-dependent CD8alpha+ and CD103+ compared with other DC subsets and immune lineages. Using a specific monoclonal antibody, we localized RAB43 expression to the Golgi apparatus and LAMP1- cytoplasmic vesicles. Mice with germline or conditional deletion of Rab43 are viable and fertile and have normal development of cDCs but show a defect for in vivo and in vitro cross-presentation of cell-associated antigen. This defect is specific to cDCs, as Rab43-deficient monocyte-derived DCs showed no defect in cross-presentation of cell-associated antigen. These results suggest that RAB43 provides a specialized activity used in cross-presentation selectively by CD8alpha+ DCs but not other antigen-presenting cells. |
