| First Author | Wu X | Year | 2016 |
| Journal | J Exp Med | Volume | 213 |
| Issue | 12 | Pages | 2553-2565 |
| PubMed ID | 27810926 | Mgi Jnum | J:237517 |
| Mgi Id | MGI:5812844 | Doi | 10.1084/jem.20160600 |
| Citation | Wu X, et al. (2016) Mafb lineage tracing to distinguish macrophages from other immune lineages reveals dual identity of Langerhans cells. J Exp Med 213(12):2553-2565 |
| abstractText | Current systems for conditional gene deletion within mouse macrophage lineages are limited by ectopic activity or low efficiency. In this study, we generated a Mafb-driven Cre strain to determine whether any dendritic cells (DCs) identified by Zbtb46-GFP expression originate from a Mafb-expressing population. Lineage tracing distinguished macrophages from classical DCs, neutrophils, and B cells in all organs examined. At steady state, Langerhans cells (LCs) were lineage traced but also expressed Zbtb46-GFP, a phenotype not observed in any other population. After exposure to house dust mite antigen, Zbtb46-negative CD64+ inflammatory cells infiltrating the lung were substantially lineage traced, but Zbtb46-positive CD64- cells were not. These results provide new evidence for the unique identity of LCs and challenge the notion that some inflammatory cells are a population of monocyte-derived DCs. |
