| First Author | Kask K | Year | 2000 |
| Journal | Brain Res | Volume | 876 |
| Issue | 1-2 | Pages | 55-61 |
| PubMed ID | 10973593 | Mgi Jnum | J:64536 |
| Mgi Id | MGI:1889458 | Doi | 10.1016/s0006-8993(00)02599-3 |
| Citation | Kask K, et al. (2000) Developmental profile of kainate receptor subunit KA1 revealed by cre expression in YAC transgenic mice. Brain Res 876(1-2):55-61 |
| abstractText | To determine the spatio-temporal expression in brain of the high-affinity kainate receptor subunit KA1, we generated transgenic mice expressing Cre recombinase from the KA1 gene on a chromosomally integrated 550 kb yeast artificial chromosome (YAC). Activity of the KA1 gene promoter during brain development was visualized by Cre immunohistochemistry, and by X-gal staining of beta-galactosidase induced by Cre recombinase in double transgenic KA1-Cre/lacZ indicator mice. During early brain development, expression from the YAC-carried KA1-Cre transgene was observed in all major brain areas, predicting a function for KA1 in the developing central nervous system. In the adult brain, KA1-Cre transgene expression was restricted mainly to hippocampal CA3 pyramidal and dentate gyrus granule cells, an adult expression pattern characteristic for the endogenous KA1 alleles. KA1-Cre transgenic mice may help in elucidating the role of floxed genes ablated in vivo in KA1 expressing neurons. |
