| First Author | Klochendler A | Year | 2016 |
| Journal | Diabetes | Volume | 65 |
| Issue | 7 | Pages | 2081-93 |
| PubMed ID | 26993067 | Mgi Jnum | J:249458 |
| Mgi Id | MGI:5923592 | Doi | 10.2337/db16-0003 |
| Citation | Klochendler A, et al. (2016) The Genetic Program of Pancreatic beta-Cell Replication In Vivo. Diabetes 65(7):2081-93 |
| abstractText | The molecular program underlying infrequent replication of pancreatic beta-cells remains largely inaccessible. Using transgenic mice expressing green fluorescent protein in cycling cells, we sorted live, replicating beta-cells and determined their transcriptome. Replicating beta-cells upregulate hundreds of proliferation-related genes, along with many novel putative cell cycle components. Strikingly, genes involved in beta-cell functions, namely, glucose sensing and insulin secretion, were repressed. Further studies using single-molecule RNA in situ hybridization revealed that in fact, replicating beta-cells double the amount of RNA for most genes, but this upregulation excludes genes involved in beta-cell function. These data suggest that the quiescence-proliferation transition involves global amplification of gene expression, except for a subset of tissue-specific genes, which are "left behind" and whose relative mRNA amount decreases. Our work provides a unique resource for the study of replicating beta-cells in vivo. |
