| First Author | Andreu MJ | Year | 2022 |
| Journal | Cell Rep | Volume | 41 |
| Issue | 3 | Pages | 111501 |
| PubMed ID | 36260992 | Mgi Jnum | J:330642 |
| Mgi Id | MGI:7380211 | Doi | 10.1016/j.celrep.2022.111501 |
| Citation | Andreu MJ, et al. (2022) Establishment of 3D chromatin structure after fertilization and the metabolic switch at the morula-to-blastocyst transition require CTCF. Cell Rep 41(3):111501 |
| abstractText | The eukaryotic genome is organized in 3D at different scales. This structure is driven and maintained by different chromatin states and by architectural factors, such as the zinc finger protein CTCF. Zygotic genome structure is established de novo after fertilization, but its impact during the first stages of mammalian development is unclear. We show that deletion of Ctcf in mouse embryos impairs the establishment of chromatin structure, but the first cell fate decision is unperturbed and embryos are viable until the late blastocyst. Furthermore, maternal CTCF is not necessary for development. Gene expression changes in metabolic and protein homeostasis programs that occur during the morula-to-blastocyst transition depend on CTCF. However, these changes do not correlate with disruption of chromatin but with binding of CTCF to the promoter of downregulated genes. Our results show that CTCF regulates both 3D genome organization and transcription during mouse preimplantation development, but as independent processes. |
