| First Author | Jin N | Year | 2007 |
| Journal | Genesis | Volume | 45 |
| Issue | 11 | Pages | 716-21 |
| PubMed ID | 17987665 | Mgi Jnum | J:129106 |
| Mgi Id | MGI:3768698 | Doi | 10.1002/dvg.20348 |
| Citation | Jin N, et al. (2007) Generation of a Mig-6 conditional null allele. Genesis 45(11):716-21 |
| abstractText | Mitogen-inducible gene 6 (Mig-6) is a stress-induced gene that serves as a negative regulator of epidermal growth factor (EGF) signaling and acts as a tumor suppressor. Ablation of Mig-6 results in a significant percentage of embryo lethality as well as abnormalities in multiple tissues. To understand the physiological roles of Mig-6, a conditional null allele, Mig-6(f/f) was generated by introducing LoxP sites that flank exons 2 and 4. The Mig-6(f/f) allele was validated by generating recombined Mig-6(-/-) mice using the Zp3-Cre system. The conditional null allele was confirmed by assaying for Mig-6 gene expression in liver, lung, uterus, and skin. The recombined Mig-6(-/-) mice developed pathological changes, such as degenerative joint diseases and skin hyperplasia similar to the previously reported Mig-6 germline null allele. In addition, these mice also had enlarged uteri with endometrial hyperplasia. In summary, this Mig-6(f/f) mouse is a useful tool for the functional study of the Mig-6 gene in a tissue-specific fashion. |
