| First Author | Okuyama M | Year | 2010 |
| Journal | Biochem Biophys Res Commun | Volume | 397 |
| Issue | 3 | Pages | 559-63 |
| PubMed ID | 20617552 | Mgi Jnum | J:162397 |
| Mgi Id | MGI:4818829 | Doi | 10.1016/j.bbrc.2010.05.157 |
| Citation | Okuyama M, et al. (2010) A novel in vivo inducible dendritic cell ablation model in mice. Biochem Biophys Res Commun 397(3):559-63 |
| abstractText | Dendritic cells (DCs) are involved in T cell activation via their uptake and presentation of antigens. In vivo function of DCs was analyzed using transgenic mouse models that express diphtheria toxin receptor (DTR) or the diphtheria toxin-A subunit (DTA) under the control of the CD11c/Itgax promoter. However, CD11c+ cells are heterogeneous populations that contain several DC subsets. Thus, the in vivo function of each subset of DCs remains to be elucidated. Here, we describe a new inducible DC ablation model, in which DTR expression is induced under the CD11c/Itgax promoter after Cre-mediated excision of a stop cassette (CD11c-iDTR). Crossing of CD11c-iDTR mice with CAG-Cre transgenic mice, expressing Cre recombinase under control of the cytomegalovirus immediate early enhancer-chicken beta-actin hybrid promoter, led to the generation of mice, in which DTR was selectively expressed in CD11c+ cells (iDTRDelta mice). We successfully deleted CD11c+ cells in bone marrow-derived DCs in vitro and splenic CD11c+ cells in vivo after DT treatment in iDTRDelta mice. This mouse strain will be a useful tool for generating mice lacking a specific subset of DCs using a transgenic mouse strain, in which the Cre gene is expressed by a DC subset-specific promoter. |
