|  Help  |  About  |  Contact Us

Publication : Pronuclear injection-based mouse targeted transgenesis for reproducible and highly efficient transgene expression.

First Author  Ohtsuka M Year  2010
Journal  Nucleic Acids Res Volume  38
Issue  22 Pages  e198
PubMed ID  20880997 Mgi Jnum  J:173808
Mgi Id  MGI:5050382 Doi  10.1093/nar/gkq860
Citation  Ohtsuka M, et al. (2010) Pronuclear injection-based mouse targeted transgenesis for reproducible and highly efficient transgene expression. Nucleic Acids Res 38(22):e198
abstractText  Mouse transgenesis has proven invaluable for analysis of gene function and generation of human disease models. We describe here the development of a pronuclear injection-based targeted transgenesis (PITT) system, involving site-specific integration in fertilized eggs. The system was applied to two different genomic target loci to generate a series of transgenic lines including fluorescent mice, which reproducibly displayed strong, ubiquitous and stable transgene expression. We also demonstrated that knockdown mice could be readily generated by PITT by taking advantage of the reproducible and highly efficient expression system. The PITT system, which circumvents the problem of unpredictable and unstable transgene expression of conventional random-integration transgenic mice, reduces the time, cost and effort needed to generate transgenic mice, and is potentially applicable to both in vivo 'gain-of-function' and 'loss-of-function' studies.
Quick Links:
 
Quick Links:
 

Expression

Publication --> Expression annotations

 

Other

22 Bio Entities

0 Expression