| First Author | Tsujita M | Year | 1999 |
| Journal | J Neurosci | Volume | 19 |
| Issue | 23 | Pages | 10318-23 |
| PubMed ID | 10575029 | Mgi Jnum | J:58752 |
| Mgi Id | MGI:1349555 | Doi | 10.1523/JNEUROSCI.19-23-10318.1999 |
| Citation | Tsujita M, et al. (1999) Cerebellar granule cell-specific and inducible expression of Cre recombinase in the mouse. J Neurosci 19(23):10318-23 |
| abstractText | To develop a cell type-specific and temporal regulation system of gene targeting in the cerebellum, we used the NMDA-type glutamate receptor GluRepsilon3 subunit gene and Cre recombinase-progesterone receptor fusion (CrePR) gene in combination. Injection of the CrePR gene placed under the control of the 10 kb 5' region of the GluRepsilon3 gene into C57BL/6 eggs yielded the ECP25 line that strongly expressed the CrePR mRNA selectively in the granule cells of the cerebellum. Using a transgenic mouse carrying a reporter gene for Cre-mediated recombination, we showed that antiprogestins could induce the recombinase activity of CrePR protein in the cerebellar granule cells of the ECP25 line. Thus, the established mouse line will provide a valuable tool to investigate the mechanism of cerebellar function by manipulating molecules in the temporally regulated and granule cell-specific manner. |
