| First Author | Dominguez MH | Year | 2023 |
| Journal | Cell | Volume | 186 |
| Issue | 3 | Pages | 479-496.e23 |
| PubMed ID | 36736300 | Mgi Jnum | J:333140 |
| Mgi Id | MGI:7434047 | Doi | 10.1016/j.cell.2023.01.001 |
| Citation | Dominguez MH, et al. (2023) Graded mesoderm assembly governs cell fate and morphogenesis of the early mammalian heart. Cell 186(3):479-496.e23 |
| abstractText | Using four-dimensional whole-embryo light sheet imaging with improved and accessible computational tools, we longitudinally reconstruct early murine cardiac development at single-cell resolution. Nascent mesoderm progenitors form opposing density and motility gradients, converting the temporal birth sequence of gastrulation into a spatial anterolateral-to-posteromedial arrangement. Migrating precardiac mesoderm does not strictly preserve cellular neighbor relationships, and spatial patterns only become solidified as the cardiac crescent emerges. Progenitors undergo a mesenchymal-to-epithelial transition, with a first heart field (FHF) ridge apposing a motile juxta-cardiac field (JCF). Anchored along the ridge, the FHF epithelium rotates the JCF forward to form the initial heart tube, along with push-pull morphodynamics of the second heart field. In Mesp1 mutants that fail to make a cardiac crescent, mesoderm remains highly motile but directionally incoherent, resulting in density gradient inversion. Our practicable live embryo imaging approach defines spatial origins and behaviors of cardiac progenitors and identifies their unanticipated morphological transitions. |
