Primary Identifier | MGI:2176512 | Allele Type | Transgenic |
Attribute String | Reporter | Gene | Tg(CAG-cat,-lacZ)11Miya |
Strain of Origin | (C57BL/6 x DBA/2)F1 | Is Recombinase | false |
Is Wild Type | false |
description | The authors state that the strain of origin is "BDF1." |
molecularNote | This allele is used to detect transient expression of Cre recombinase. The transgene carries a lacZ gene downstream of a CMV enhancer and chicken beta-actin promoter and a floxed chloramphenicol acetyltransferase gene (a stuffer sequence). Upon Cre-mediated excision of the stuffer sequence, the modified allele expresses lacZ in tissues where Cre is expressed. The authors state that a single copy of the transgene was inserted and expression was observed throughout all embryonic and adult stages. |