| First Author | Imayoshi I | Year | 2012 |
| Journal | Neurosci Res | Volume | 73 |
| Issue | 1 | Pages | 85-91 |
| PubMed ID | 22343123 | Mgi Jnum | J:182745 |
| Mgi Id | MGI:5316535 | Doi | 10.1016/j.neures.2012.02.003 |
| Citation | Imayoshi I, et al. (2012) A multifunctional teal-fluorescent Rosa26 reporter mouse line for Cre- and Flp-mediated recombination. Neurosci Res 73(1):85-91 |
| abstractText | Reporters of Cre and/or Flp activity are important for defining the spatial and temporal extent of Cre/Flp-mediated recombination. Here, we describe R26-CAG-LF-mTFP1, a multifunctional fluorescent reporter mouse that strongly expresses mTFP1 (bright teal fluorescent protein) after Cre- and Flp-mediated recombination. To meet the need for single recombinase-mediated reporter expression, we generated derivatives of R26-CAG-LF-mTFP1. The germline excision of the Frt-flanked stop cassette in R26-CAG-LF-mTFP1 generated a Cre-dependent reporter (R26-CAG-LoxP-mTFP1). Similarly, R26-CAG-FRT-mTFP1, in which the loxP-flanked stop cassette was excised in the germline, requires only Flp to activate mTFP1 expression. |
