| First Author | González-Tajuelo R | Year | 2020 |
| Journal | Front Immunol | Volume | 11 |
| Pages | 588212 | PubMed ID | 33281818 |
| Mgi Jnum | J:337296 | Mgi Id | MGI:6729371 |
| Doi | 10.3389/fimmu.2020.588212 | Citation | Gonzalez-Tajuelo R, et al. (2020) Relevance of PSGL-1 Expression in B Cell Development and Activation. Front Immunol 11:588212 |
| abstractText | PSGL-1 is expressed in all plasma cells, but only in a small percentage of circulating B cells. Patients with systemic sclerosis (SSc) show reduced expression of PSGL-1 in B cells and increased prevalence of pulmonary arterial hypertension. PSGL-1 deficiency leads to a SSc-like syndrome and SSc-associated pulmonary hypertension in female mice. In this work, the expression of PSGL-1 was assessed during murine B cell development in the bone marrow and in several peripheral and spleen B cell subsets. The impact of PSGL-1 absence on B cell biology was also evaluated. Interestingly, the percentage of PSGL-1 expressing cells and PSGL-1 expression levels decreased in the transition from common lymphoid progenitors to immature B cells. PSGL-1(-/-) mice showed reduced frequencies of peripheral B cells and reduced B cell lineage-committed precursors in the bone marrow. In the spleen of WT mice, the highest percentages of PSGL-1(+) populations were shown by Breg (90%), B1a (34.7%), and B1b (19.1%), while only 2.5-8% of B2 cells expressed PSGL-1; however, within B2 cells, the class-switched subsets showed the highest percentages of PSGL-1(+) cells. Interestingly, PSGL-1(-/-) mice had increased IgG(+) and IgD(+) subsets and decreased IgA(+) population. Of note, the percentage of PSGL-1(+) cells was increased in all the B cell subclasses studied in peritoneal fluid. Furthermore, PSGL-1 engagement during in vitro activation with anti-IgM and anti-CD40 antibodies of human peripheral B cells, blocked IL-10 expression by activated human B cells. Remarkably, PSGL-1 expression in circulating plasma cells was reduced in pulmonary arterial hypertension patients. In summary, although the expression of PSGL-1 in mature B cells is low, the lack of PSGL-1 compromises normal B cell development and it may also play a role in the maturation and activation of peripheral naive B cells. |
