| First Author | Turner NL | Year | 2022 |
| Journal | Cell | Volume | 185 |
| Issue | 6 | Pages | 1082-1100.e24 |
| PubMed ID | 35216674 | Mgi Jnum | J:322765 |
| Mgi Id | MGI:7259977 | Doi | 10.1016/j.cell.2022.01.023 |
| Citation | Turner NL, et al. (2022) Reconstruction of neocortex: Organelles, compartments, cells, circuits, and activity. Cell 185(6):1082-1100.e24 |
| abstractText | We assembled a semi-automated reconstruction of L2/3 mouse primary visual cortex from approximately 250 x 140 x 90 mum(3) of electron microscopic images, including pyramidal and non-pyramidal neurons, astrocytes, microglia, oligodendrocytes and precursors, pericytes, vasculature, nuclei, mitochondria, and synapses. Visual responses of a subset of pyramidal cells are included. The data are publicly available, along with tools for programmatic and three-dimensional interactive access. Brief vignettes illustrate the breadth of potential applications relating structure to function in cortical circuits and neuronal cell biology. Mitochondria and synapse organization are characterized as a function of path length from the soma. Pyramidal connectivity motif frequencies are predicted accurately using a configuration model of random graphs. Pyramidal cells receiving more connections from nearby cells exhibit stronger and more reliable visual responses. Sample code shows data access and analysis. |
