| First Author | Shimizu T | Year | 2017 |
| Journal | Biochem Biophys Res Commun | Volume | 493 |
| Issue | 2 | Pages | 946-951 |
| PubMed ID | 28943044 | Mgi Jnum | J:251048 |
| Mgi Id | MGI:6103015 | Doi | 10.1016/j.bbrc.2017.09.115 |
| Citation | Shimizu T, et al. (2017) Prolyl isomerase Pin1 promotes proplatelet formation of megakaryocytes via tau. Biochem Biophys Res Commun 493(2):946-951 |
| abstractText | Here we show that Pin1, a peptidyl-prolyl cis/trans isomerase which catalyzes the isomerization of phosphorylated Ser/Thr-Pro, is a regulatory molecule of thrombopoiesis. We found that mice lacking the Pin1 gene (Pin1(--) mice) formed more megakaryocytes (MKs) than wild type mice (WT mice), and that the proplatelet formation of MKs was poorer in Pin1(--) mice than WT mice. Treatment of Meg-01 cells, a megakaryoblastic floating cell line, with shRNA against Pin1 suppressed the proplatelet formation. Expression of tau, a microtubule associated protein was induced in MKs during proplatelet formation. Pin1 bound tau and promoted microtubule polymerization. Our results show that Pin1 serves as a positive regulatory molecule of proplatelet formation of MKs by enhancing the function of phosphorylated tau. |
