| First Author | Pradhan J | Year | 2011 |
| Journal | Biochem Biophys Res Commun | Volume | 405 |
| Issue | 2 | Pages | 157-61 |
| PubMed ID | 21215724 | Mgi Jnum | J:168459 |
| Mgi Id | MGI:4888416 | Doi | 10.1016/j.bbrc.2010.12.127 |
| Citation | Pradhan J, et al. (2011) Glycine-induced currents are insensitive to the glycine receptor alpha(1) subunit-specific blocker, cyanotriphenylborate, in older circling mice. Biochem Biophys Res Commun 405(2):157-61 |
| abstractText | The pharmacologic characteristics of glycine receptors (GlyRs) in the lateral superior olive (LSO) of circling mice, animal model for inherited deafness, were investigated using a GlyR alpha(1) subunit-specific receptor blocker (cyanotriphenylborate [CTB]). There was a statistically significant age-dependent increase in the antagonistic effect of CTB in heterozygous (+/cir) mice. In postnatal (P)0-P3 heterozygous (+/cir) mice, glycine currents evoked by glycine puffs were reduced to 20.4+/-2.6, 37.1+/-3.1, and 63.9+/-2.5% at 0.1, 1, and 10muM CTB (n=13) compared to controls, while the glycine currents were reduced to 22.3+/-3.5, 52.9+/-4.1, and 78.3+/-3.5% at 0.1, 1, and 10muM CTB (n=7) in P8-P12 heterozygous (+/cir) mice. In contrast, the antagonistic effect of CTB was not strong and even less than that of younger animals in older homozygous (cir/cir) mice. In P0-P3 homozygous (cir/cir) mice, the extent of inhibition was 20.2+/-3.7, 37.8+/-4.3, and 66.8+/-4.2% at 0.1, 1, and 10muM CTB (n=6) compared to controls, while the extent of inhibition was 18.7+/-2.4, 28.1+/-3.9, and 39.1+/-8.2% (n=6) in P8-P12 homozygous (cir/cir) mice. The age-dependent decrease in the antagonistic effect of CTB indicates the abnormal development of the alpha(1) subunit-containing GlyRs in homozygous (cir/cir) mice. |
