| First Author | Chen S | Year | 2004 |
| Journal | Biochem Biophys Res Commun | Volume | 324 |
| Issue | 1 | Pages | 147-53 |
| PubMed ID | 15464995 | Mgi Jnum | J:93166 |
| Mgi Id | MGI:3056045 | Doi | 10.1016/j.bbrc.2004.08.232 |
| Citation | Chen S, et al. (2004) Effects of flanking genes on the phenotypes of mice deficient in basigin/CD147. Biochem Biophys Res Commun 324(1):147-53 |
| abstractText | The induction of null mutations by means of homologous recombination is a powerful technique for clarifying the biological activities of target genes. However, the problems of the genetic background and flanking genes should be borne in mind. Here we employed a breeding strategy to compare three lines of mice deficient in the basigin (Bsg)/CD147 gene. The first line was F2 from F1 hybrid offspring of the 129/SV chimera and C57BL/6J. The second one was from a C57BL/6J congenic line. Both lines showed high embryonic lethality, sterility, and blindness. The third one was 'reverse F2' from 'reverse F1' hybrid offspring of the C57BL/6J congenic line and 129/SV. Surprisingly, this line showed a normal birth rate, while sterility and blindness persisted. Our results clearly separate the effects of the induced null mutation from those of flanking genes and the genetic background, and provide a useful means of investigating the biological functions of Bsg. |
