| First Author | Melis E | Year | 2005 |
| Journal | Biochem Biophys Res Commun | Volume | 333 |
| Issue | 2 | Pages | 488-95 |
| PubMed ID | 15961065 | Mgi Jnum | J:99260 |
| Mgi Id | MGI:3581907 | Doi | 10.1016/j.bbrc.2005.05.144 |
| Citation | Melis E, et al. (2005) Thrombophilia in mice expressing a tissue factor variant lacking its transmembrane and cytosolic domain. Biochem Biophys Res Commun 333(2):488-95 |
| abstractText | Mice with a targeted truncation in the gene encoding tissue factor of blood coagulation (TF) to eliminate the cytosolic domain and carrying a neo(R) cassette in intron 5 unexpectedly displayed severe spontaneous thrombosis in various vascular beds. Thrombosis was observed in heterozygous TF(+/neo) mice, causing death of over 50% of adults within 36 weeks of birth, and fulminantly exacerbating in pregnant females. Homozygous TF(neo/neo) mice were more severely affected and died within 7 weeks after birth. These TF(neo) mice primarily synthesized a mutant mRNA aberrantly spliced from exon 5 to neo(R), encoding an apparently non-vesicle-binding soluble TF lacking both the transmembrane and cytosolic domain, but still capable of blood coagulation induction. This severe thrombotic phenotype associated with the presence of a non-anchored soluble TF variant underscores the recently recognized significance of circulating TF for thrombus formation and development. |
