| First Author | Pan L | Year | 2002 |
| Journal | J Immunol | Volume | 168 |
| Issue | 8 | Pages | 3923-32 |
| PubMed ID | 11937548 | Mgi Jnum | J:75746 |
| Mgi Id | MGI:2177721 | Doi | 10.4049/jimmunol.168.8.3923 |
| Citation | Pan L, et al. (2002) An Analysis of T Cell Intrinsic Roles of E2A by Conditional Gene Disruption in the Thymus. J Immunol 168(8):3923-32 |
| abstractText | The importance of E2A transcription factors in T cell development has been demonstrated in studies of E2A-deficient mice, which display abnormal T cell development and a high frequency of T cell lymphomas. Because E2A expression is not restricted to the T cell lineage, the primary cause of the T cell phenotype in E2A-deficient mice was not fully determined. To further investigate the role of E2A in T cell lineage, we generated mice with the E2A gene disrupted exclusively during thymocyte development using the Cre-lox system. We show that this system allows E2A gene disruption to occur throughout the double-negative stage of thymocyte development. E2A deletion appears to be completed before development reaches the double-positive stage. Consistent with the gene disruption, these mice reveal a T cell intrinsic role for E2A during the transition from the double-negative stage to the double-positive stage of thymocyte development. In contrast to germline E2A knockout mice, conditional E2A knockout mice do not develop T cell lymphoma. This work establishes a new model for further investigating E2A function in T cell development and leukemiogenesis. |
