| First Author | Bounds HA | Year | 2023 |
| Journal | Cell Rep | Volume | 42 |
| Issue | 8 | Pages | 112909 |
| PubMed ID | 37542722 | Mgi Jnum | J:339333 |
| Mgi Id | MGI:7519963 | Doi | 10.1016/j.celrep.2023.112909 |
| Citation | Bounds HA, et al. (2023) All-optical recreation of naturalistic neural activity with a multifunctional transgenic reporter mouse. Cell Rep 42(8):112909 |
| abstractText | Determining which features of the neural code drive behavior requires the ability to simultaneously read out and write in neural activity patterns with high precision across many neurons. All-optical systems that combine two-photon calcium imaging and targeted photostimulation enable the activation of specific, functionally defined groups of neurons. However, these techniques are unable to test how patterns of activity across a population contribute to computation because of an inability to both read and write cell-specific firing rates. To overcome this challenge, we make two advances: first, we introduce a genetic line of mice for Cre-dependent co-expression of a calcium indicator and a potent soma-targeted microbial opsin. Second, using this line, we develop a method for read-out and write-in of precise population vectors of neural activity by calibrating the photostimulation to each cell. These advances offer a powerful and convenient platform for investigating the neural codes of computation and behavior. |
