| First Author | Kolobarić N | Year | 2023 |
| Journal | Int J Mol Sci | Volume | 24 |
| Issue | 8 | PubMed ID | 37108475 |
| Mgi Jnum | J:335264 | Mgi Id | MGI:7468105 |
| Doi | 10.3390/ijms24087315 | Citation | Kolobaric N, et al. (2023) Tff3(-/-) Knock-Out Mice with Altered Lipid Metabolism Exhibit a Lower Level of Inflammation following the Dietary Intake of Sodium Chloride for One Week. Int J Mol Sci 24(8) |
| abstractText | A high salt intake causes hemodynamic changes and promotes immune response through cell activation and cytokine production, leading to pro-inflammatory conditions. Transgenic Tff3(-/-) knock-out mice (TFF3ko) (n = 20) and wild-type mice (WT) (n = 20) were each divided into the (1) low-salt (LS) group and (2) high-salt (HS) group. Ten-week-old animals were fed with standard rodent chow (0.4% NaCl) (LS) or food containing 4% NaCl (HS) for one week (7 days). Inflammatory parameters from the sera were measured by Luminex assay. The integrin expression and rates of T cell subsets of interest from the peripheral blood leukocytes (PBLs) and mesenteric lymph nodes (MLNs) were measured using flow cytometry. There was a significant increase in high-sensitivity C reactive protein (hsCRP) only in the WT mice following the HS diet, while there were no significant changes in the serum levels of IFN-gamma, TNF-alpha, IL-2, IL-4, or IL-6 as a response to treatment in either study groups. The rates of CD4(+)CD25(+) T cells from MLNs decreased, while CD3(+)gammadeltaTCR(+) from peripheral blood increased following the HS diet only in TFF3ko. gammadeltaTCR expressing T cell rates decreased in WT following the HS diet. The CD49d/VLA-4 expression decreased in the peripheral blood leukocytes in both groups following the HS diet. CD11a/LFA-1 expression significantly increased only in the peripheral blood Ly6C(-)CD11a(high) monocytes in WT mice following salt loading. In conclusion, salt-loading in knock-out mice caused a lower level of inflammatory response compared with their control WT mice due to gene depletion. |
