| Primary Identifier | MGI:2179227 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Lig1 |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd-Hprt1<b-m3> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The 3' end of the gene (exons 23-27) was replaced with a PGK-HPRT minigene. Northern analysis failed to detect any intact, truncated, or fusion transcripts in homozygous mutant embryos. Western analysis failed to detect any protein in homozygous mutant embryos, when probed with antibodies against bovine DNA ligase 1. |
