| First Author | Requardt RP | Year | 2009 |
| Journal | Glia | Volume | 57 |
| Issue | 6 | Pages | 680-92 |
| PubMed ID | 18942753 | Mgi Jnum | J:156217 |
| Mgi Id | MGI:4419065 | Doi | 10.1002/glia.20796 |
| Citation | Requardt RP, et al. (2009) Quality control of astrocyte-directed Cre transgenic mice: the benefits of a direct link between loss of gene expression and reporter activation. Glia 57(6):680-92 |
| abstractText | Cre recombinase activity for cell-type restricted deletion of floxed target genes (i.e., flanked by Cre recognition loxP-sites) is often measured by separate matings with recombination-activated reporter gene mice. Using a floxed Gja1 (Cx43) allele, we demonstrate the benefits of a direct link between reporter gene expression and target gene deletion to overcome critical limitations of the Cre/loxP system. The widely used human glial fibrillary acidic protein (hGFAP)-Cre transgene exhibits variable recombination activity and requires postexperimental validation. Such quality control is essential to correlate the extent of Cre-mediated Gja1 ablation with phenotypical alterations and to maintain the activity status of hGFAP-Cre in transgenic mouse colonies. We present several strategies to control for the fidelity of hGFAP-Cre mediated recombination. (c) 2008 Wiley-Liss, Inc. |
