| Primary Identifier | MGI:2179724 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Mapkapk2 |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The catalytic domain of this gene, encompassing subdomains V and VI, was replaced with a neomycin resistance cassette via homologous recombination which introduced stop codons in all 3 reading frames and results in a truncated kinase lacking amino acids 130-383. Northern blot of spleen from homozygous mutant animals verified the absence of gene expression and kinase assay of mouse embryonic fibroblasts verified the absence of enzyme activity. |
