| First Author | Hannigan MO | Year | 2001 |
| Journal | J Immunol | Volume | 167 |
| Issue | 7 | Pages | 3953-61 |
| PubMed ID | 11564814 | Mgi Jnum | J:118560 |
| Mgi Id | MGI:3699773 | Doi | 10.4049/jimmunol.167.7.3953 |
| Citation | Hannigan MO, et al. (2001) Abnormal migration phenotype of mitogen-activated protein kinase-activated protein kinase 2-/- neutrophils in Zigmond chambers containing formyl-methionyl-leucyl-phenylalanine gradients. J Immunol 167(7):3953-61 |
| abstractText | Time-lapsed video microscopy and confocal imaging were used to study the migration of wild-type (WT) and mitogen-activated protein kinase-activated protein kinase 2 (MK2-/-) mouse neutrophils in Zigmond chambers containing fMLP gradients. Confocal images of polarized WT neutrophils showed an intracellular gradient of phospho-MK2 from the anterior to the posterior region of the neutrophils. Compared with WT neutrophils, MK2-/- neutrophils showed a partial loss of directionality but higher migration speed. Immunoblotting experiments showed a lower protein level of p38 mitogen-activated protein kinase and a loss of fMLP-induced extracellular signal-related kinase phosphorylation in MK2-/- neutrophils. These results suggest that MK2 plays an important role in the regulation of neutrophil migration and may also affect other signaling molecules. |
