| Primary Identifier | MGI:2180148 | Allele Type | Targeted |
| Gene | Syt1 | Transmission | Germline |
| Strain of Origin | 129P2/OlaHsd | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | Insertion of a neomycin resistance cassette into intron 1 and an arginine to glutamine point mutation at position 233 of exon 1 (R233Q) was introduced via homologous recombination. Western blot of brain homogenates from homozygous mutant animals verified the presence of protein expression and enzyme assays demonstrated similar phospholipid- and syntaxin-binding ability compared to wild-type. However, calcium affinity was decreased by 2-fold. |
