| First Author | Moin AS | Year | 2012 |
| Journal | Biochem Biophys Res Commun | Volume | 428 |
| Issue | 4 | Pages | 512-7 |
| PubMed ID | 23111332 | Mgi Jnum | J:191092 |
| Mgi Id | MGI:5460942 | Doi | 10.1016/j.bbrc.2012.10.073 |
| Citation | Moin AS, et al. (2012) Neuroendocrine regulatory peptide-2 stimulates glucose-induced insulin secretion in vivo and in vitro. Biochem Biophys Res Commun 428(4):512-7 |
| abstractText | Neuroendocrine regulatory peptide (NERP)-2, recently identified as a bioactive peptide involved in vasopressin secretion and feeding regulation in the central nervous system, is abundantly expressed in endocrine cells in peripheral tissues. To explore the physiological roles of NERP-2 in the pancreas, we examined its effects on insulin secretion. NERP-2 increased glucose-stimulated insulin secretion (GSIS) in a dose-dependent manner, with a lowest effective dose of 10(-7) M, from the pancreatic beta-cell line MIN6 and isolated mouse pancreatic islets. NERP-2 did not affect insulin secretion under the low-glucose conditions. Neither NERP-1 nor NERP-2-Gly (nonamidated NERP-2) stimulated insulin secretion. NERP-2 significantly augmented GSIS after intravenous administration to anesthetized rats or intraperitoneal injection to conscious mice. We detected NERP-2 in pancreatic islets, where it co-localized extensively with insulin. Calcium-imaging analysis demonstrated that NERP-2 increased the calcium influx in MIN6 cells. These findings reveal that NERP-2 regulates GSIS by elevating intracellular calcium concentrations. |
