| First Author | Cretney E | Year | 2003 |
| Journal | J Exp Med | Volume | 198 |
| Issue | 3 | Pages | 491-6 |
| PubMed ID | 12900523 | Mgi Jnum | J:84838 |
| Mgi Id | MGI:2670315 | Doi | 10.1084/jem.20030634 |
| Citation | Cretney E, et al. (2003) Normal thymocyte negative selection in TRAIL-deficient mice. J Exp Med 198(3):491-6 |
| abstractText | The molecular basis of thymocyte negative selection, which plays a critical role in establishing and maintaining immunological tolerance, is not yet resolved. In particular, the importance of the death receptor subgroup of the tumor necrosis factor (TNF)-family has been the subject of many investigations, with equivocal results. A recent report suggested that TRAIL was a critical factor in this process, a result that does not fit well with previous studies that excluded a role for the FADD-caspase 8 pathway, which is essential for TRAIL and Fas ligand (FasL) signaling, in negative selection. We have investigated intrathymic negative selection of TRAIL-deficient thymocytes, using four well-established models, including antibody-mediated TCR/CD3 ligation in vitro, stimulation with endogenous superantigen in vitro and in vivo, and treatment with exogenous superantigen in vitro. We were unable to demonstrate a role for TRAIL signaling in any of these models, suggesting that this pathway is not a critical factor for thymocyte negative selection. |
