| Primary Identifier | MGI:2179992 | Allele Type | Transgenic |
| Attribute String | Inducible, Inserted expressed sequence | Gene | Tg(tetO-Kras2)12Hev |
| Strain of Origin | FVB/N | Induced With | doxycycline/tetracycline |
| Is Recombinase | false | Is Wild Type | false |
| description | Homozygous transgenic mice are viable and fertile. In conjunction with a second transgene encoding either rtTA (reverse tetracycline trans-activator protein) or tTA (the tetracycline trans-activator), mice carrying Tg(tetO-Kras2)12Hev show tissue-specific expression of the mutated K-ras transgene that may be induced or suppressed by administration of the tetracycline analog, doxycycline. Cessation or initiation of doxycycline treatment in bitransgenic strains containing, respectively, rtTA or tTA, will result in a rapid decrease of expression of the transgene. |
| molecularNote | The transgene expresses the mutant gene Kras4bG12D, under the control of a tetracycline-responsive promoter element (tTA), mp-1 intron polyadenylation site sequence, and a minimal CMV promoter. |
