| First Author | Sordella R | Year | 2002 |
| Journal | Dev Cell | Volume | 2 |
| Issue | 5 | Pages | 553-65 |
| PubMed ID | 12015964 | Mgi Jnum | J:76484 |
| Mgi Id | MGI:2179574 | Doi | 10.1016/s1534-5807(02)00162-4 |
| Citation | Sordella R, et al. (2002) Modulation of CREB Activity by the Rho GTPase Regulates Cell and Organism Size during Mouse Embryonic Development. Dev Cell 2(5):553-65 |
| abstractText | Rho GTPases regulate several aspects of tissue morphogenesis during animal development. We found that mice lacking the Rho-inhibitory protein, p190-B RhoGAP, are 30% reduced in size and exhibit developmental defects strikingly similar to those seen in mice lacking the CREB transcription factor. In p190-B RhoGAP-deficient mice, CREB phosphorylation is substantially reduced in embryonic tissues. Embryo-derived cells contain abnormally high levels of active Rho protein, are reduced in size, and exhibit defects in CREB activation upon exposure to insulin or IGF-1. The cell size defect is rescued by expression of constitutively activated CREB, and in wild-type cells, expression of activated Rho or dominant-negative CREB results in reduced cell size. Together, these results suggest that activity of the Rho GTPase modulates a signal from insulin/IGFs to CREB that determines cell size and animal size during embryogenesis. |
