| First Author | Murakami M | Year | 2007 |
| Journal | Biochem Biophys Res Commun | Volume | 354 |
| Issue | 4 | Pages | 1016-20 |
| PubMed ID | 17275790 | Mgi Jnum | J:120350 |
| Mgi Id | MGI:3706302 | Doi | 10.1016/j.bbrc.2007.01.087 |
| Citation | Murakami M, et al. (2007) Modified sympathetic regulation in N-type calcium channel null-mouse. Biochem Biophys Res Commun 354(4):1016-20 |
| abstractText | To elucidate the physiological importance of neuronal (N)-type calcium channels in sympathetic controls, we analyzed N-type channel-deficient (NKO) mice. Immunoprecipitation analysis revealed increased interaction between beta3 (a major accessory subunit of N-type channels) and R-type channel-forming CaV2.3 in NKO mice. R-R intervals in NKO ECG recordings were elongated and fluctuating, suggesting disturbed sympathetic tonus. N-type channel inhibitors elongated the R-R interval in control mice, whereas R-type channel blocking with SNX-482 significantly affected NKO but not control mice, indicating a compensatory role for R-type channels. Echocardiography and Langendorff heart analysis confirmed a major role for R-type channels in NKO mice. Combined, our biochemical and physiological analyses strongly suggest that the remaining sympathetic tonus in NKO mice is dependent on R-type calcium channels. |
