| First Author | Meyer RA | Year | 1994 |
| Journal | Endocrine | Volume | 2 |
| Issue | 12 | Pages | 1127-1132 |
| Mgi Jnum | J:26058 | Mgi Id | MGI:73680 |
| Citation | Meyer RA, et al. (1994) Measurement of parathyroid hormone in the mouse: secondary hyperparathyroidism in the X-linked hypophosphatemic (Gyro, gy) mouse. Endocrine 2(12):1127-1132 |
| abstractText | X-linked hypophosphatemia is a metabolic bone disease of humans (the HYP gene) and mice (the Hyp and Gy genes). Secondary hyperparathyroidism is a feature of the Hyp mouse and some human patients. In this study, we evaluated the rat PTH (IRMA) kit (Immutopics, San Clemente, CA) for mice. Acute EDTA injection (0.2 g/kg) ip resulted in decreased serum calcium and increased serum PTH (42+/-10 vs. 250+/-41 pg/ml, vehicle vs. EDTA, mean+/-SE, P<0.01) 20 min after treatment. Conversely, chronic infusion of 1,25-dihydroxyvitamin D-3 (150 ng/kg/day) resulted in hypercalcemia and reduced serum PTH (20.6+/-3.5 vs. 8.7+/- 2.2 pg/ml, P<0.05). Parathyroidectomy also decreased serum PTH levels. Serum of EDTA-treated mice was diluted and gave PTH values which paralleled the standard curve. Thus, this kit gave valid measurements of PTH in mice. Intact Gy mice were slightly hypocalcemic. After parathyroidectomy (PTX), the Gy mice remained hypocalcemic relative to PTX normal mice (1.83+/-0.03 vs. 1.51+/-0.05 mM, P<0.01). Serum PTH in untreated Gy mice was significantly higher than in normal mice (18.1+/-1.2 vs. 40.8+/-4.5 pg/ml, P<0.01). Hypocalcemia in intact and PTX Gy mice and increased serum PTH suggest the presence of secondary hyperparathyroidism in the Gy mice. |
