| First Author | Ohno H | Year | 1993 |
| Journal | EMBO J | Volume | 12 |
| Issue | 11 | Pages | 4357-66 |
| PubMed ID | 8223445 | Mgi Jnum | J:15271 |
| Mgi Id | MGI:63399 | Doi | 10.1002/j.1460-2075.1993.tb06120.x |
| Citation | Ohno H, et al. (1993) Developmental and functional impairment of T cells in mice lacking CD3 zeta chains. EMBO J 12(11):4357-66 |
| abstractText | CD3 zeta is a component of the T cell antigen receptor (TCR) complex and is important for signal transduction. We have established mice selectively lacking CD3 zeta but able to express CD3 eta, a polypeptide produced from the same locus through alternative splicing, using the method of gene targeting in embryonic stem cells. In homozygous mutant mice, the numbers of thymocytes and peripheral T cells were greatly reduced and the expression levels of TCR on these cells were 5-fold lower than those on wild-type cells. By contrast, TCR gamma delta+ intestinal intraepithelial lymphocytes were not obviously affected by the mutation. T cells from homozygous mutants exhibited an impaired proliferative response. These results imply that CD3 zeta has a critical role in the development and signal transduction of T cells in vivo. |
