| First Author | Arase H | Year | 2001 |
| Journal | J Immunol | Volume | 166 |
| Issue | 1 | Pages | 21-5 |
| PubMed ID | 11123272 | Mgi Jnum | J:66403 |
| Mgi Id | MGI:1928424 | Doi | 10.4049/jimmunol.166.1.21 |
| Citation | Arase H, et al. (2001) Negative regulation of expression and function of FcgammaRIII by CD3zeta in murine NK cells. J Immunol 166(1):21-5 |
| abstractText | FcgammaRIII is involved in Ab-dependent cell-mediated cytotoxicity (ADCC) and cytokine production by NK cells. Signaling and expression of FcgammaRIII are dependent on FcRgamma. Although NK cells express not only FcRgamma but also CD3zeta, the role of CD3zeta in NK cell function remains unclear. Here, we found that the expression of FcgammaRIII on NK cells from CD3zeta-deficient mice is unexpectedly up-regulated compared with that on cells from normal mice. Furthermore, ADCC and IFN-gamma production upon FcgammaRIII-cross-linking by NK cells from CD3zeta-deficient mice were also up-regulated. Up-regulation of the surface expression of FcgammaRIII on CD3zeta-deficient NK cells is not mediated by transcriptional augmentation of either FcgammaRIII or FcRgamma gene because there was no significant difference in the expression of mRNA for FcgammaRIII and FcRgamma. Transfection of CD3zeta into a cell line expressing FcgammaRIII and FcRgamma induced a decrease in the cell surface expression of FcgammaRIII. These findings reveal a negative regulatory role of CD3zeta in FcgammaRIII-mediated function of murine NK cells. |
