| Primary Identifier | MGI:2180488 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Bhlhe40 |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A neomycin resistance gene under the control of a GTI-II promoter replaced a region encoding the first 6 amino acids, including the initiator-methionine, and a portion of the proximal promoter. Northern blot analyis confirmed a lack of transcript in heart, liver, and kidney tissue obtained from homozygous mutant mice. An absence of protein expression was determined homozygous mutant MEFs via Western blot analysis. |
